Evolutionary, Molecular and Genetic Analyses of Tic22 Homologues in Arabidopsis thaliana Chloroplasts
Figure 6
Chloroplast protein levels in the tic22 double mutants.
A. Equal protein samples (20 µg) from 5-day-old homozygous, MS-grown plants of the indicated genotypes were analysed by immunoblotting using the antibodies shown. LHCP, light-harvesting chlorophyll-binding protein; OE33, oxygen-evolving complex 33 kD subunit; FNR, ferredoxin-NADP reductase; CPO, coproporphyrinogen oxidase; H3, histone H3. B,C. Chloroplast protein bands in A (as well as those from other, similar experiments) were quantified using Aida software (Raytest), and then the data were normalized using equivalent H3 data. Values shown are means (±SE) derived from 3–8 independent measurements.