Glioma Cell Death Induced by Irradiation or Alkylating Agent Chemotherapy Is Independent of the Intrinsic Ceramide Pathway
Figure 6
Exogenous ceramide is cytotoxic to human glioma cell lines and reduces clonogenic survival.
Acute cytotoxicity was assessed 72 h after treatment with increasing concentrations of (A) C2-ceramide or (B) C6-ceramide in LNT-229 or T98G cells. For clonogenic survival data LNT-229 or T98G cells were treated with increasing concentrations of (C) C2-ceramide or (D) C6-ceramide for 24 h, then medium was changed and cells were cultured for additional 2 weeks until colonies were detectable. *, p<0.05; **, p<0.01; compared to untreated cells; t-test.