Transcriptome Analysis of Buds and Leaves Using 454 Pyrosequencing to Discover Genes Associated with the Biosynthesis of Active Ingredients in Lonicera japonica Thunb.
Figure 4
Proposed pathways for the biosynthesis of CGA and luteoloside in L. japonica.
The three different routes of CGA biosynthesis are labeled 1, 2, and 3. Enzyme names are shown in the pictures. Each enzyme is annotated with the number of corresponding unigenes shown in parentheses. PAL, phenylalanine ammonia lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-hydroxycinnamoyl CoA ligase/4-coumarate-CoA ligase; HCT, hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyltransferase; C3H, p-coumarate 3′-hydroxlase; HQT, hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase; UGCT, UDP glucose: cinnamate glucosyl transferase; HCGQT, hydroxycinnamoyl D-glucose: quinate hydroxycinnamoyl transferase. CHS, chalcone synthesis; CHI, chalcone isomerase; FSII, flavonol synthase; F3'H, Flavonoid 3'-monooxygenase.