Hypoxic Preconditioning with Cobalt of Bone Marrow Mesenchymal Stem Cells Improves Cell Migration and Enhances Therapy for Treatment of Ischemic Acute Kidney Injury
Figure 9
HMP-MSC injection protected the kidney from I/R injury better than NP-MSC administration.
Renal function was evaluated by determining (A) Scr and (B) BUN levels in the week following I/R AKI. Administration of HMP-MSC had a better maintained renal function than NP-MSC and vehicle treatment at 24 h and 1 wk following transplantation. (C) Renal morphological changes after I/R injury (H&E-stainning, magnification ×200). (D) Tubulointerstitial (TI) injury score in the cortex and outer medullar region. HMP-MSC administration significantly lowered TI injury score at 24 h and 1 wk after reperfusion. (E) HMP-MSC rats showed lower numbers of CD68 positive macrophages than medium and NP-MSC groups at 24 h and 1 wk after reperfusion. (F) TUNEL positive apoptotic cells in the three groups were not significantly different at 24 h after injection, but it was significantly lower in HMP-MSC group than in medium and NP-MSC groups 1 week after injection. (G) The number of Ki-67 positive proliferating cells was higher in HMP-MSC group at 24 h after injection with most of these cells localized in tubules (Figure 10). Conversely, the number of Ki-67 positive cells was lower in HMP-MSC group 1 week after injection with most of the remaining located in interstitium (Figure 10). *P<0.05, vs medium; #P<0.05, vs NP-MSC.