Natural Killer Cell Function, an Important Target for Infection and Tumor Protection, Is Impaired in Type 2 Diabetes
Figure 5
Tunicamycin induces ER stress and UPR activation, and decreases NKG2D expression in normal PBMCs in vitro.
PBMCs from healthy donors were incubated in the absence (Ctrl) or in the presence (Tm) of 1.25 µg/mL tunicamycin for 6 hours. (A) ER stress markers BiP, HERP, GRP94 and PDI, (B) IRE1α pathway marker spliced XBP1 (sXBP1) and (C) PERK pathway markers ATF4, GADD34 and CHOP mRNA amounts were determined by quantitative RT-PCR in normal PBMCs. Transcript levels are presented as mean±SEM (n = 6). *:P<0.05. (D) NKG2D, NKp46 and NKG2C expressions were quantified by flow cytometry (n = 12). *:P<0.05.