Mouse Transplant Models for Evaluating the Oncogenic Risk of a Self-Inactivating XSCID Lentiviral Vector
Figure 3
Recipient origin of T-cell malignancies arising in the secondary recipients of the EF1a group in experiment 2.
Tumor cells derived from the spleen of transplanted mice were analyzed for CD4 and CD8 expression first (top panels). The abnormal CD4+ CD8+ leukemic cells were then gated and analyzed for CD45.1 (donor) and CD45.2 (recipient) marker expression. Virtually all CD4+CD8+ cells exclusively expressed CD45.2 and were therefore derived from the irradiated recipient mice.