Low Cost Extraction and Isothermal Amplification of DNA for Infectious Diarrhea Diagnosis
Figure 4
Gel electrophoresis analysis of the HDA on-chip amplicons using 12% polyacrylamide gel with MspI digested pBR322 as marker.
(A) five positive human stool DNA samples infected with C. difficile; (B) 3 negative human stool samples; (C) HDA in-tube as a control, and PCR control reactions to quantify the amount of DNA in the stool extract using human GAPDH as a housekeeping gene.