The E. coli Effector Protein NleF Is a Caspase Inhibitor
Figure 6
Caspase binding and apoptosis inhibition by modified versions of NleF.
A–C. Caspase-9-binding of different versions of NleF assessed by LUMIER assays. A. Caspase-4, B. caspase-8, C. caspase-9. D. Percentage of apoptotic HeLa cells expressing different versions of NleF after induction with staurosporine. NleF +1: NleF with an additional C-terminal alanine; NleF -1 and NleF -4: NleF with the terminal 1 and 4 amino acids removed, respectively; NleF L186A, NleF Q187A, NleF C188A and NleF G189A: NleF versions with indicated amino acids substituted by alanine; NleF +18: NleF with additional 18 amino acids.