Metabolic Adaptation to Chronic Inhibition of Mitochondrial Protein Synthesis in Acute Myeloid Leukemia Cells
Figure 8
Changes in mitochondrial morphology and HIF1α expression in RTEX-TIG cells are irreversible. A
DNA was extracted from TEX, RTEX+TIG cells, and RTEX-TIG cells. Quantitative PCR was performed for mitochondrial ND1 relative to the human globulin gene (HGB). Results shown as mean ± SD ratio of ND1/HGB compared to TEX cells from a representative experiment done in triplicate. B Mitochondrial morphology (arrows) was assessed in TEX, RTEX+TIG and RTEX-TIG cells using transmission electron microscopy as described in the Materials and Methods section. Representative images taken at 50,000× are shown. The scale bar is 500 nm. C Total cellular RNA was isolated and HIF1α expression was measured relative to 18S RNA by real-time RT-PCR. Data represent the mean±SD HIF1α/18S expression relative to TEX cells.