Cell Wall Assembly and Intracellular Trafficking in Plant Cells Are Directly Affected by Changes in the Magnitude of Gravitational Acceleration
Figure 4
Intracellular trafficking is reduced at hyper-gravity.
Geometry (A,B) and fluorescence intensity (A,C) of the apical vesicle cone. Vesicles were labelled with the styryl dye FM1–43 taken up by endocytosis. At the time of imaging the dye had been incorporated into most of the endomembrane system including exocytotic vesicles. (A) represents a pollen tube marked with FM1–43, the orange arrow represents the diameter of the pollen tube. Surface area of orthographic projection of the vesicle cone (B) and fluorescence intensity (area 1 in A) as well as the fluorescence intensity of the distal cytoplasm (area 2 in A) were measured in pollen tubes grown at 1 g and in hyper-g conditions. Relative fluorescence intensity (C) was expressed as a ratio between the fluorescence of the vesicle cone over the fluorescence of the distal cytoplasm fluorescence of the same tube. Asterisks (*) indicate statistically significant difference between samples grown in hyper-g and control samples grown at 1 g (Two way t-test yielded p<5.10−4 for the three graphs). Bar = 10 µm.