Concordant and Discordant Regulation of Target Genes by miR-31 and Its Isoforms
Figure 3
The isomiRs of miR-31 display differential ability in repressing Dicer expression.
Immunoblotting of Dicer in MCF-7 cells transfected with 100 nM synthetic oligos purchased from Ambion (A) and Dharmacon (B). Immunoblotting of Dicer in MDA-MB-231 cells (C), in A549 cells (D), and in HCT116 (E) transfected with 100 nM synthetic oligos (Ambion). Relative expression of Dicer mRNA in MCF-7 cells transfected with miRNA synthetic oligos (Ambion) (F). Data were presented as relative expression level to Neg-ctrl transfectant. Neg-ctrl, negative control oligo.