In Cellulo Examination of a Beta-Alpha Hybrid Construct of Beta-Hexosaminidase A Subunits, Reported to Interact with the GM2 Activator Protein and Hydrolyze GM2 Ganglioside
Figure 1
Western blot analysis of the lysate from wild-type (WT) human fibroblasts (positive control), untransfected (UT) Feline SD fibroblasts and human TSD Glial cells (negative control), and a clonal population of the indicated cell types highly expressing either the H1 or H2 beta-alpha hybrid subunit (Table 1) or the WT alpha subunit.
The positions of the alpha (αp) and beta (βp) precursor polypeptides and the mature (lysosomal) alpha (αm) and beta (βm) chains are indicated. Note that both hybrids lack the internal posttranslational cleavage site present in the beta, but not the alpha subunit, that has been linked to GM2AP binding.