Characterisation of K+ Channels in Human Fetoplacental Vascular Smooth Muscle Cells
Figure 7
mRNA and protein expression of KCa channels in CPAs.
Isolated CPASMCs expressed BKCa (A) and IKCa (B) protein. BKCa (D) and IKCa (E) protein was localised to the SMCs in intact CPA sections. Negative control (C) and (F); substitution of primary antibody with non-immune IgG. RT-PCR detection of BKCa (G) IKCa (H) and SKCa3 (I) transcripts in human reference cDNA (Std), chorionic plate artery (CPA). Negative (Neg) is omission of cDNA. Amplicon lengths; BKCa 147, IKCa 102 and SKCa3 106. SMC; smooth muscle cell, L; lumen.