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Modulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria Diet

Figure 5

Effect of primary antibody dilution on representative Western blots of rat plasma CBG of male and female rats subjected to 30 d of cafeteria diet compared with controls.

Abbreviations for the groups are the same as in Figure 1. Numbers represent the titer of the primary antibody (1) and the secondary (2). The conditions for standard Western blot used (as in Figure 4) were those of the upper photograph. In all cases, the range of MW for the spots was between 50,000 and 75,000 according to the scale (not shown, since it could be seen only in the membrane).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0057342.g005