Expression of Fungal Cutinase and Swollenin in Tobacco Chloroplasts Reveals Novel Enzyme Functions and/or Substrates
Figure 1
Evaluation of transgene integration and homoplasmy by Southern blot analysis.
A, Schematic representation of the chloroplast transformation vectors pLD-swo1 and pLD-cut. Prrn, rRNA operon promoter; aadA, aminoglycoside 3′-adenylyltransferase gene; 5′ UTR, promoter and 5′ untranslated region of psbA gene; 3′ UTR, 3′ untranslated region of psbA gene. B, Southern blot analysis of independent cutinase transplastomic lines hybridized with chloroplast flanking sequence probe showing heteroplasmy (UT, untransformed; T1–T3, transplastomic lines). C, Southern blot analysis of independent swollenin transplastomic lines hybridized with chloroplast flanking sequence probe showing homoplasmy (UT, untransformed; T1–T4, transplastomic lines).