Adhesion of Annexin 7 Deficient Erythrocytes to Endothelial Cells
Figure 6
Correlation between phosphatidylserine exposure and dynamic adhesion of erythrocytes to endothelial cells under arterial shear stress.
Arithmetic means ± SEM (n = 6−8) of the erythrocytes adhering to HUVEC as a function of the percentage erythrocytes binding annexin V. The erythrocytes from annexin7-deficient mice (anx7−/−, closed symbols) and their wild type control mice (anx7+/+, open symbols) were left without pretreatment (circles), or pretreated 30 minutes with 1 µM ionomycin (triangles), 12 hours with glucose-depleted Ringer (squares) or 2 hours with hyperosmotic shock by addition of 550 mM sucrose (diamonds).