FCHSD1 and FCHSD2 Are Expressed in Hair Cell Stereocilia and Cuticular Plate and Regulate Actin Polymerization In Vitro
Figure 3
FCHSD1 and FCHSD2 immunolocalization in mouse cochlear hair cells.
Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. (A) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse cochlear hair cells. (B) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) FCHSD2 immunoreactivity in the hair bundles of 6-week old mouse cochlear hair cells. (D) FCHSD2 immunoreactivity in the hair bundles of 7-month old mouse inner hair cells. (E) FCHSD2 immunoreactivity in the hair bundles of 2-week old mouse outer hair cells. The Novus FCHSD1 antibody was used in (A) and (B). Scale bars: 10 µm in(A-C), 5 µm in (D) and (E).