MICU2, a Paralog of MICU1, Resides within the Mitochondrial Uniporter Complex to Regulate Calcium Handling
Figure 3
MICU1 and MICU2 can be silenced in vivo in mouse liver using siRNA technology.
A. In vitro dose-response curves of selected duplexes targeting MICU1 and MICU2. B. Relative expression of MICU1 and MICU2 mRNA after 6 weekly injections normalized to siLUC mice. C. Representative oxygen consumption traces measured in isolated mitochondria from siLUC (top) and siMICU1+2 (bottom) mice. Arrows denote addition of mitochondria, glutamate and malate (G/M), ADP and uncoupler (carbonyl cyanide m-chlorophenylhydrazone, CCCP). Respiratory control ratios (RCR) and ADP∶O ratios (P∶O) were calculated from experiments performed on three separate mice per group. D. Representative mitochondrial membrane potential traces measured in isolated mitochondria from siLUC (top) and siMICU1+2 (bottom) mice using tetramethyl rhodamine methyl ester (TMRM). E. Respiratory control ratios (RCR) and ADP∶O ratios (P∶O) were comparable among all treatment groups.