Human Alveolar Epithelial Cells Attenuate Pulmonary Microvascular Endothelial Cell Permeability under Septic Conditions
Figure 1
Dose-dependent effects of cytomix and lipopolysaccharide (LPS) on human pulmonary microvascular endothelial cell (PMVEC) permeability.
PMVEC seeded on cell-culture inserts for 5–6 days formed a monolayer, which was subsequently stimulated with either cytomix (A) or LPS (B) vs PBS for 8-hours, and the permeability determined by measurement of trans-PMVEC leak of Evans blue (EB) dye-labeled albumin over the final 1-hour. The dose-response effects of cytomix and LPS were both significant (P<0.01) by ANOVA; *: P<0.05 vs. PBS/non-stimulated groups.