Both OsRecQ1 and OsRDR1 Are Required for the Production of Small RNA in Response to DNA-Damage in Rice
Figure 1
The detection of aRNAs and qiRNAs in WT and the OsRecQ1 mutant line (ND8004) after the DNA damaging agent UV or EMS treatment by northern blot and RT-qPCR analysis.
(A) The results show a significant induction of qiRNAs about 20–21 nucleotides (nt) in length after UV or EMS treatments in WT, but a complete abolishment in the OsRecQ1 mutant line. An RNA probe derived from the sense 25S rDNA region was used. The arrow denotes the qiRNAs. The bottom panel of tRNAs shows equal loading control. (B) The results show a marked induction of 25S rDNA specific transcripts after UV or EMS treatments in WT, but a complete loss in the OsRecQ1 mutant line (ND8004). An RNA probe derived from sense 25S rDNA region was used. The bottom panel of rRNAs shows equal loading control. (C) A schematic diagram shows the upstream (U) and downstream (D) primers from rice rDNA regions for RT-qPCR analysis. The transcriptional start site is shown with an arrow. (D) RT-qPCR results indicating an abolishment of aRNAs from the rDNA locus induced by UV treatment in the OsRecQ1 mutant lines (ND8004). The expressing level of rice ubiquitin gene was used as the internal control. Two independent experiments are shown. Data are the mean ± SE (n = 3), *P<0.001. RT-qPCR analysis showing a loss of aRNAs from the rDNA locus induced by EMS treatment in the OsRecQ1 mutant lines (ND8004).