T-Type Ca2+ Current Activity during Oocyte Growth and Maturation in the Ascidian Styela plicata
Figure 9
Effect of Ca2+ current inhibition on the in vitro fertilization and embryo development in Styela plicata.
A) Slightly deformed embryos at 2-cell stage, developed from oocytes fertilized in ASW containing 25 µM NiCl2, arrested at an abnormal 8-cell stage; B) Abnormal hatched larvae developed from embryos transferred at 2-cell stage in ASW containing 25 µM NiCl2. Bar is 50 µm in A and 100 µm in B.