An Improved Genotyping by Sequencing (GBS) Approach Offering Increased Versatility and Efficiency of SNP Discovery and Genotyping
Figure 6
Impact of selective amplification on the number and depth of coverage of SNPs.
(a) Schematic representation of an ApeKI restriction fragment flanked by suitable ligated adapters and the position of standard or selective primers, (b) Comparison of the number of SNPs and sequence read depth obtained with different sets of primers, and (c) number of SNPs and mean depth of coverage observed with selective amplification of ApeKI digested fragments with AC selective primers at different levels of multiplexing.