Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
Figure 3
Differentiation of hMSC to adipocytes and osteoblasts.
Representative images for Oil O Red and Alizarin red staining demonstrating adipocyte and osteoblasts differentiation of hMSC cell lines cultured under adipogenic and osteogenic conditions, respectively. Non-differentiated cells were not stained (A and D), while the adipocyte differentiated cells were stained with Oil O red (B) and Alizarin red stained the matrix formed by osteoblasts (E). qPCR data shows marked up-regulation of PPAR gamma gene expression after adipocyte differentiation (C) and up-regulation of Collagen 1 gene expression in osteoblast differentiated cells (F).