MicroRNA-22 (miR-22) Overexpression Is Neuroprotective via General Anti-Apoptotic Effects and May also Target Specific Huntington’s Disease-Related Mechanisms
Figure 2
miR-22 is neuroprotective against mutant Htt.
Primary striatal neurons A) or cortical neurons B) were infected on DIV1 with lentiviral vectors encoding wild-type (Htt18Q) or mutant (Htt82Q) Htt171 fragments under the control of the TRE promoter. On DIV4, where denoted, cultures were infected with vector encoding miR-22. Neuronal viability was assessed after 3 weeks in culture by quantification of NeuN-positive cells. Graphs represent mean ± SEM, n = 15. ** represents p-value <0.01.