SALL4 Expression in Gonocytes and Spermatogonial Clones of Postnatal Mouse Testes
Figure 7
Differential expression of GFRα1 and SALL4 in As and Apr spermatogonia.
Expression of GFRα1 was limited to short chains of Aundiff and more restricted than expression of SALL4. (A–D) Examples of GFRα1 and SALL4 expressing spermatogonial clones in whole mount seminiferous tubules. Scale bars = 10 µm. Panel D was modified from [1] with permission. (E) Quantitative evaluation of GFRα1 and SALL4 expression in clones of undifferentiated spermatogonia. Among As spermatogonia, 50% of cells co-expressed both markers, but substantial populations of SALL4-only and GFRα1-only cells were also observed. The number of SALL4-only cells increased in longer chains, when GFRα1 expression ceased. Asym: Number of clones with molecular asymmetry.