In Vivo Targeting of ADAM9 Gene Expression Using Lentivirus-Delivered shRNA Suppresses Prostate Cancer Growth by Regulating REG4 Dependent Cell Cycle Progression
Figure 6
Decreased REG4 and increased p21Cip1/WAF1, p27Kip1, and cyclin D1 expression in ADAM9 knockdown prostate cancer cells.
(a) Quantitative PCR confirmed the decrease of REG4 mRNA and increase of CD33 mRNA. (b) Overexpression of REG4 decreased p21Cip1/WAF1 expression under FBS starvation conditions in PC3shADAM9. (c) The starvation-induced increase in superoxide levels was reversed by overexpression of REG4 in PC3shADAM9. (d) The strongest inhibition of REG4 expression by shREG4 lentiviral vectorwas observed in PC3shREG4-225663 by RT-PCR. (e) PC3shREG4-225663 reduced the S-phase and increased the G1-phase populations under starvation conditions.