RING Finger Protein 11 Targets TBK1/IKKi Kinases to Inhibit Antiviral Signaling
Figure 5
RNF11 requires TAX1BP1 to inhibit antiviral signaling.
(A) 293T cells were transfected with IFN-β luciferase reporter (200 ng), pRL-tk (20 ng), RNF11 (1 µg) and either control scrambled or TAX1BP1 siRNA (60 pmol). After 24 h, cells were transfected with poly(I:C) (15 µg) and dual luciferase assays were performed 16 h later. The data is presented as percent inhibition by RNF11 of poly(I:C)-induced IFN-β promoter induction with either control scrambled or TAX1BP1 siRNA (left panel). Knockdown of TAX1BP1 was confirmed by immunoblotting using anti-TAX1BP1 and anti-Actin (right panel). (B) Tax1bp1+/– and Tax1bp1–/– MEFS were transfected with poly(I:C) (20 μg), and co-IPs were performed with anti-RNF11 followed by immunoblotting with anti-IKKi (left panel) or anti-TBK1 (right panel). Immunoblots were performed with lysates using anti-IKKi, anti-RNF11, anti-TBK1 and anti-Actin. *, p<0.05. Error bars, S.D.