Effects of Initial Cell Density and Hydrodynamic Culture on Osteogenic Activity of Tissue-Engineered Bone Grafts
Figure 4
Proliferation of seeded cells in cell-scaffold constructs was detected by cell counting kit-8 (A) and osteoblastic differentiation of seeded cells in cell-scaffold constructs was evaluated by ALP activities (B).
The number of cells was increased with culture time except group C. The dynamic culture (groups A and B) showed an obvious ability of promoting proliferation of cells. The ALP activities in all groups increased from day 2 to day 14 (B). The ALP activities in groups A, B, D were statistically higher than that in groups C(p<0.05) from day 4 to day 14. indicates a statistically higher value compared with group C(p<0.05).