Temperature-Dependent Structural Changes of Parkinson's Alpha-Synuclein Reveal the Role of Pre-Existing Oligomers in Alpha-Synuclein Fibrillization
Figure 1
High temperature pretreatment inhibits α-synuclein fibril formation.
(A) α-Synuclein (25 µM) was subjected to 1 hr pretreatment in 20 (•), 40 (▴), 60 (▪), 80 (⋄), and 95 (▹) °C and the fibril formation was monitored at room temperature by addition of 20 µM ThT with continuous shaking at 1,000 rpm for 10 days. (B) Western blotting of the end-point products of α-synuclein in ThT assay. The pretreated temperatures and the molecular weight markers are indicated.