Profiling of Androgen Response in Rainbow Trout Pubertal Testis: Relevance to Male Gonad Development and Spermatogenesis
Figure 2
Expression of androgen-responsive and developmentally-regulated genes.
Heatmap representation of specific expression clusters of androgen-regulated genes that also display differential expression during trout spermatogenesis. Subgroups of genes were established by crossing 4 clusters (1 to 4) of androgen-responsive genes and 9 clusters (A to I) of spermatogenesis as indicated in table 1. For these genes, we present expression signals obtained: - in testes from controls and androgen-supplemented animals, and - in testes at various developmental stages and in enriched fractions of isolated germ cells. Normalized expression values are shown according to the scale bar while histograms represent averaged fold changes to the control (androgens data) or to the stage I (spermatogenesis data) ± SEM for each cluster. T1 and T2 correspond to animals supplemented with testosterone implants of 0.1 and 0.2 mg, respectively; 11KT indicates animals treated with 11-ketotestosterone implants of 0.25 mg; d7 and d14 correspond to animals treated for 7 and 14 days, respectively. Roman numerals (I–V and VIII) indicate testicular developmental stages. Sg, Sc and St correspond to isolated germ cell populations enriched in spermatogonia, spermatocytes and spermatids, respectively. The annotation of each gene is accessible in the searchable File S1.