Clk/STY (cdc2-Like Kinase 1) and Akt Regulate Alternative Splicing and Adipogenesis in 3T3-L1 Pre-Adipocytes
Figure 6
Single Akt-site mutations on Clk1 dysregulate PKCβII and increase Akt phosphorylation of SRp40.
A. 3T3-L1 cells were transfected with single mutant Clk1 constructs as indicated and PKCβII levels, phosphoAkt-substrate for SRp40 and SRp40 levels were probed as indicated in cell lysates collected from cells exposed to differentiation conditions. B. 3T3-L1 pre-adipocytes were transfected with Clk1-AAA mutant and probed for phosphorylation of SRp40 by Clk1 using mAb104, phosphoAkt substrate antibody to detect Akt phosphorylation of SRp40, and SRp40 antibody in cell lysates from cells exposed to differentiation conditions. Transfections were performed on multiple occasions to ensure reproducible results.