Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV
Figure 8
Concentration of HIV-Gag to sites of cell-cell contacts.
(A–C) HEK293 cells were co-transfected with HIVNL4-3 and fluorescently tagged HIVNL4-3-GFP (green) or HIVNL4-3-RFP (red) and co-cultured with the dye-labeled target T cells MT4 cells (CFSE, green)(A), Jurkat cells (CMTPX, red))(B), and SupT1 cells (CFSE, green)(C) and imaged by confocal fluorescence microscopy. Size bars represent 10 µm.