Lithium Controls Central Nervous System Autoimmunity through Modulation of IFN-γ Signaling
Figure 4
IFN-γ-signaling is required for attenuation of EAE by lithium.
EAE was induced in (A) WT (B), Stat1−/−, (C) Ifngr1−/−, and (D) Ifnar1−/−as described in Material and Methods. Arrows indicate first day of administration of lithium. (Mean ± SEM, n = 10–13 mice/group *p<0.05, or NS, not significant, from start of treatment until day 30, as determined by Mann-Whitney test). (E) RNA was isolated from spinal cords of WT and Ifngr1−/− mice on day 20 post-immunization and evaluated for gene expression by real-time PCR, as detailed in Materials and Methods. n = 3 for immunized, n = 1 for unimmunized controls. *p<0.05, as determined by one-way ANOVA.