Long-Term In Vivo Imaging of Multiple Organs at the Single Cell Level
Figure 6
Visualizing radiation-induced thymocyte apoptosis in ear-thymus graft.
A nude BALB/c chimera containing DsRed+ hematopoietic cells were transplanted with a thymus from a B6 CD45.1 neonatal mouse (<48 hours old) into the ear pinna. Five weeks later, the ear pinna containing thymus graft was irradiated with 8.5 Gy. Cell apoptosis was then followed over time by in vivo two-photon imaging after injection of FAM-FLIVO. The images were taken 113 to 137 µm deep from surface. Representative pictures from each group are shown. The percentages of apoptotic cells are shown. Similar experiments have been repeated for three times. Green = EGFP; red = apoptotic cells. Scale bar = 50 µm.