Activity of 25-Hydroxylase in Human Gingival Fibroblasts and Periodontal Ligament Cells
Figure 5
The efficiency of RNA interference against CYP27A1 and CYP2R1.
hGF and hPDLC from donors 2, 4 and 5 were transfected with a siRNA oligonucleotide for CYP27B1, a siRNA oligonucleotide for CYP2R1, or a non-silencing control. Using real-time PCR as a measure, the efficiency of RNA interference against CYP27A1 and CYP2R1 was over 70% in hGF and hPDLC. The data are presented as the mean ± SD. * denotes difference from negative controls (p<0.05).