Non-Traditional Antibacterial Screening Approaches for the Identification of Novel Inhibitors of the Glyoxylate Shunt in Gram-Negative Pathogens
Figure 1
P. aeruginosa glyoxylate shunt mutants are deficient for growth both in vitro and in vivo.
(A) The ability of wild-type P. aeruginosa PAO1 and its isogenic glyoxylate shunt mutants to utilize various sole carbon sources was assessed spectrophotometrically after overnight growth at 37°C. (B) The ability of these strains to colonize and persist in a murine lung model of infection was measured at 2- and 48-hours post-infection by lung homogenization and subsequent CFU ml−1 determination. NR – no recoverable colonies.