Cocaine Enhances HIV-1 Replication in CD4+ T Cells by Down-Regulating MiR-125b
Figure 9
Cocaine regulates transcription of miR-125b.
(A) A schematic representation of miR-125b promoter construct used. In this construct the miR-125b promoter drives the luciferase gene. (B) A construct containing miR-125b promoter driven luciferase gene or the vector control were transfected into 293T cells by Lipofectamine transfection. After 24 hrs, these cells were treated with cocaine (0.1 µM and 1 µM) for 4 hrs. Thereafter, cells were lysed and luciferase activity was measured by a luminometer. Cocaine treatment decreased miR-125b driven luciferase expression. Data are representative of three independent experiments conducted in triplicates.