Molecular Cloning and Characterization of Novel Morus alba Germin-Like Protein Gene Which Encodes for a Silkworm Gut Digestion-Resistant Antimicrobial Protein
Figure 2
Analytical HPLC profile of purified novel protein found to have antibacterial activity.
Peak ‘A’ fractions obtained from Gel filtration chromatography was analyzed using the reverse-phase C18 column (4.6×250 mm) and eluted with 30–70% acetonitrile – 0.05% TFA linear gradient mode with a flow rate of 1.0 ml/min and detection wavelength of 280 nm. A single peak (denoted by arrow) with a retention time of 12.95 min was observed. The area percent for the peak was 100 and area was 73412.