HMGB in Mollusk Crassostrea ariakensis Gould: Structure, Pro-Inflammatory Cytokine Function Characterization and Anti-Infection Role of Its Antibody
Figure 5
mRNA expression levels of Ca-HMGB in hemocyte monolayers at different times after RLO/LPS incubation.
Samples were collected at 0, 1.5, 4, 8 and 12 h after RLO/LPS incubation. Expression levels were determined by real-time RT-PCR. The seawater-incubated groups were used as controls. Expression levels were assessed using 28SrDNA gene for normalization. *p≤0.05 when compared to control value. (A) RLO(1ul/106 cells) incubation; (B) LPS (100 ng/ml)incubation.