Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells
Figure 3
Protection against high glucose-induced oxidative stress by melatonin.
(A) Measurement of cellular glutathione contents in Müller cells exposed to high glucose. Cells were treated with 30 mM glucose (HG) alone or in together with 0.1 mM melatonin (Mel), 50 µM luzindole (Luz) or 50 µM LY294002 (Ly2). Glutathione was measured by the DTNB method. (B) Upregulation of GCLc and GCLm measured by real-time RT-PCR and normalized to β-actin mRNA. (C) Western blot analyses of HO-1 expression after co-treatment with high glucose and indicated concentrations of melatonin (Mel). (D) Effects of luzindole on melatonin-induced upregulation of HO-1. Data presented are average from 3 to 5 experiments (*P<0.05; **P<0.01).