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Requirement of Plasminogen Binding to Its Cell-Surface Receptor α-Enolase for Efficient Regeneration of Normal and Dystrophic Skeletal Muscle

Figure 5

Inhibitors of α-enolase/plasminogen binding impede skeletal muscle regeneration after injury.

A. Hematoxylin/eosin staining of cross-sections of gastrocnemius muscles of mice treated with inhibitors (MAb11G1, EACA or control) at different days post injury (d.p.i.) B. Plasmin specific activity (S.A.) of gastrocnemius muscle extracts, expressed as mOD/min/mg protein, as mean +/− SEM (error bars), * P<0.01. C. Percentage of degeneration area, percentage of central nucleated fibers (CNF) and mean fiber size of inhibitors-treated mice at 21 d.p.i., mean +/− SEM (error bars), ** P<0.005, * P<0.01. D. Western blotting of myogenin, α-enolase and β-enolase protein in tibialis anterioris muscle lysates. A representative image of triplicates is shown. Densitometric quantification of protein levels normalized with respect to α-tubulin is reported in the graphical diagrams. E. Trichrome staining for collagen deposits in gastrocnemius muscles, treated with the different inhibitors. Bars, 50 µm. n = 6 animals per group.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0050477.g005