Temporal Colonic Gene Expression Profiling in the Recurrent Colitis Model Identifies Early and Chronic Inflammatory Processes
Figure 8
Schematic representation of the colitis induction protocol.
Induction of colitis is achieved by dorsal skin application of TNBS in ethanol on day 0, followed by rectal TNBS/ethanol instillations on day 7, 14, and 21. The necropsy time-points for study 1, i.e. TNBS disease induction and corticosteroid treatment, and study 2, i.e. the time-course experiment, are presented in the upper part of the schematic. Severity of colitis induction was assessed by measurement of colon weight, length and thickness and by histopathological evaluation of the distal part of the colon. At each necropsy, serum was collected for cytokine profiling and colon tissue was sampled for RNA isolation.