Direct and Dynamic Detection of HIV-1 in Living Cells
Figure 1
Identification and in vitro characterization of a CA-specific nanobody.
(A) Amino acid sequence of CANTDcb1. (B) CANTDcb1 was tested for antigen specificity by solid phase phage ELISA, showing significant binding response for full-length CA and CANTD but no binding to CACTD. Purified GFP was used as negative control. Bars show representative values of technical duplicates. (C) Affinity measurements with a quartz crystal microbalance (QCM) system. Data fitting (red lines) and rate constant calculation was carried out with ClampXP analysis software. (D) To further measure and confirm binding specificity, purified full-length CA, CANTD or CACTD were injected at 10 µg/ml and passed over immobilized CANTDnb1.