Secretory Vesicles Are Preferentially Targeted to Areas of Low Molecular SNARE Density
Figure 3
tSNARE molecular dynamics in living membranes.
(A) Compilation of tracks from individual molecules of SNAP-25 with a temporal resolution of 50 ms(left). The indicated region (yellow box) was selected and segmented in to an array of 100 nm × 100 nm boxes. The number of tracks passing through each box was measured and is shown as a contour map of normalized density (center) such that the sum of all density values is equal to one. The mean speed of all tracks passing through each sampling box was also measured and is displayed as a contour map (right). (B) As in (A) but for sptPALM of syntaxin.