Evaluation of the Use of the Polyubiquitin Genes, Ubi4 and Ubi10 as Reference Genes for Expression Studies in Brachypodium distachyon
Figure 5
Real time PCR standard curves for the primer sets used.
(A) Standard curve generated using a serial dilution of pGEM T-Easy-Ubi10 as template. The Cp values obtained at each concentration for Ubi10Fw and Ubi10Rv [5] are shown as white circles. The Cp values obtained at each concentration for Ubi10-3Fw and Ubi10-3Rv are shown as black circles. The ΔCp between both sets of primers at each concentration is indicated by triangle. Error bars are shown for each point. (B) Standard curve generated using a serial dilution of pGEM T-Easy-Ubi4 as template. The Cp values obtained at each concentration for Ubi4Fw and Ubi4Rv [5] are shown as white circles. The Cp values obtained at each concentration for Ubi4-3Fw and Ubi4-3Rv are shown as black circles. The ΔCp between both sets of primers at each concentration is indicated by triangle. Values are mean ± SE (n = 3).