Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors
Figure 5
Expresssion of key growth factors and receptors in human blastocysts.
Cryopreserved human day 5 embryos were used for quantitative RT-PCR analyses of transcript levels for different ligand-receptor pairs (EGF/EGFR, IGF-I/IGF-IR, GM-CSF/GM-CSFR, BDNF/TrkB, CSF-1/CSF-1R, artemin/GFRA3, and GDNF/GFRA1). Levels of all mRNAs were normalized based on those for β-actin in the same sample (mean ± SEM, n = 3).