Intense Resistance Exercise Induces Early and Transient Increases in Ryanodine Receptor 1 Phosphorylation in Human Skeletal Muscle
Figure 2
Distribution and localization of total sarcoplasmic RyR1 and total sarcoplasmic AMPK in type I and II skeletal muscle myofibers.
Illustration of DAB stained myofiber cross-sections (20x magnification) showing (A) total AMPK, (B) pAMPKThr172 and (C) corresponding MyHC1 staining displaying type I myofibers (black) and type II myofibers (unstained). No fiber type-specific differences in total AMPK were observed; Illustration of DAB stained consecutive myofiber cross-sections showing (D) total RyR1, (E) pRyR1Ser2843 and (F) corresponding MyHC1 staining displaying type I myofibers (black) and type II myofibers (unstained). As displayed in (D, F) total RyR1 density was considerably lower in type I compared to type II myofibers. (G) Assessment of total RyR1 density in type I and II myofibers at baseline, 15 min, 30 min and 60 min post resistance exercise. Type II myofibers displayed significantly higher RyR1 densities than type I myofibers (p<0.01). Total RyR1 did not change over the time-course in type I and II myofibers. *headed bars display significant differences between myofibers types (p<0.01).