Label-Free Single-Particle Imaging of the Influenza Virus by Objective-Type Total Internal Reflection Dark-Field Microscopy
Figure 3
Correlation between scattering and fluorescence images of influenza viruses obtained with TIRDFM and TIRFM.
(A) Scattering image. (B) Fluorescence image. (C) False-color superimposed image of binarized scattering (red) and fluorescence (green) images shown in A and B. (D) Distribution of the distance between scattering (fluorescence) spots and nearest-neighbor fluorescence (scattering) spots. We considered scattering and fluorescence spots within 400 nm (dashed line) as signals from the same particle.