Interaction of Inflammation and Hyperoxia in a Rat Model of Neonatal White Matter Damage
Figure 2
Inflammation and hyperoxia decreases MBP expression.
A significantly decrease of MBP protein expression as a sign of hypomyelination occurs in all experimental settings with similar severity. Results of densitometric western blot quantification of P3 LPS P6 hyperoxia experiment (A), results of densitometric western blot quantification of P6 LPS P6 hyperoxia experiment (B), a representative western blot series of P3 LPS P6 hyperoxia experiment (C), a representative western blot series of P3 LPS P6 hyperoxia experiment (D) and images of immunohistological stained sections against MBP (E) are shown. Immunohistological staining against MBP supports these findings and illustrates a profound reduction of MBP in external capsule with loss of processes and thinning up to fragmentation of the capsule in all treated groups. Scale bar = 100 µm. Brain hemisphere extracts were used for western blot analyses with n = 9–11 animals per group. Western blot values represent mean+SEM normalized ratios of the MBP bands to β-actin as an internal standard and VN group was set to 100%. * p<0.05, ** p<0.01, *** p<0.001 and ns p>0.05; VN: vehicle+normoxia, LN: LPS+normoxia, VH: vehicle+hyperoxia, LH: LPS+hyperoxia.