Lentiviral Transduction of CD34+ Cells Induces Genome-Wide Epigenetic Modifications
Figure 8
Illumina Infinium analysis also detects distinct methylation patterns in the CD34+ cells depending on the stimulation and/or lentiviral transduction.
A. Dendogram obtained by unsupervised hierarchical clustering of 12 samples (triplicates for each condition) analysed. The LV transduced cells (Group 4) clusters separately of all others (upper part). The heat-map columns under the squares with the sample number represent the methylation level (beta values) of the genes that changed by at least by 20% between the experimental conditions. B. Comparison of the numbers of genes that increased (red) or decreased (green) methylation between the groups. The number of methylated/demethylated genes increases dramatically in LV transduced as compared to the two the non-transduced CD34+ cells.